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Mouse IL-6 Quantikine ELISA Kit
PM6000B 1 Pack (50 Plates) / Please Inquire
Cell Culture Supernates, Serum, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 28 | 22 | 27 |
| Mean | 30 | 87 | 232 | 29 | 85 | 238 |
| Standard Deviation | 2 | 3.4 | 8.1 | 1.8 | 7.5 | 18.1 |
| CV% | 6.7 | 3.9 | 3.5 | 6.1 | 8.9 | 7.6 |
The recovery of mouse IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 110 | 86-120 |
| Heparin Plasma (n=6) | 101 | 81-115 |
| Serum (n=5) | 99 | 84-113 |