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主营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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  • 注册时间:2018-04-28
  • 经营模式:生产型,
  • 所在地区:广东省 深圳市
  • 联系人:苏元元女士
  • 手 机:18665335907
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  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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  • Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit
  • Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit

  • 发布时间:2021-11-01 11:41:37
  • 关键词:Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit
  • 产品描述:Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit
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Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary

Catalog              Size / Price
MLB00C              1 Kit (2 Plates) / Please Inquire
SMLB00C             1 Pack (6 Plates) / Please Inquire

PMLB00C             1 Pack (50 Plates) / Please Inquire

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
4.8 pg/mL
Assay Range
12.5 - 800 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Mouse IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 beta in cell culture supernates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IL-1 beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 beta.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 33.5 92.9 386 34.6 93.5 423
Standard Deviation 2.5 4.3 11.7 2.9 6.2 23.9
CV% 7.5 4.6 3 8.4 6.6 5.7

Recovery

The recovery of mouse IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 106 95-119
EDTA Plasma (n=4) 108 101-117
Heparin Plasma (n=4) 97 87-114
Serum (n=4) 105 98-113
Tissue Lysates (n=4) 115 110-120

深圳子科生物科技有限公司
  • 联系人:苏元元女士
  • 手 机:18665335907
  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

深圳子科生物科技有限公司 地址:广东省 深圳市 深圳龙华民治 技术支持:商铺管理入口 | 产品索引 | 免责声明