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Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit
PMLB00C 1 Pack (50 Plates) / Please Inquire
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 33.5 | 92.9 | 386 | 34.6 | 93.5 | 423 |
| Standard Deviation | 2.5 | 4.3 | 11.7 | 2.9 | 6.2 | 23.9 |
| CV% | 7.5 | 4.6 | 3 | 8.4 | 6.6 | 5.7 |
The recovery of mouse IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 106 | 95-119 |
| EDTA Plasma (n=4) | 108 | 101-117 |
| Heparin Plasma (n=4) | 97 | 87-114 |
| Serum (n=4) | 105 | 98-113 |
| Tissue Lysates (n=4) | 115 | 110-120 |