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Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
PMJE00 1 Pack (50 Plates) / Please Inquire
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 47.1 | 311 | 768 | 43.6 | 310 | 783 |
| Standard Deviation | 3.9 | 15.8 | 40.6 | 3.2 | 14.3 | 42.8 |
| CV% | 8.3 | 5.1 | 5.3 | 7.3 | 4.6 | 5.5 |
The recovery of MCP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=6) | 104 | 91-119 |
| Mouse Serum (n=9) | 99 | 89-113 |
| Rat Cell Culture Supernates (n=4) | 109 | 103-115 |
| Rat Serum (n=4) | 109 | 103-116 |