Human HGF Quantikine ELISA Kit【行情报价价格评测】-企讯网

深圳子科生物科技有限公司

会8年
说明
- 会会员认证：网站通过企讯网认证，真实合法，放心访问；
- 官网认证：网站通过工信部备案，真实合法，放心访问；
- 工商认证：企业通过工商注册，营业执照真实有效；
- 加V认证：企业通过百度信誉认证，百度优先赔付；
- 诚信通认证：企业在阿里巴巴诚信通有资料，已经开通阿里巴巴旺铺；
- 微店认证：企业开通朝阳微商城，可以通过移动网络购买商品。
- 认证或商铺装修请联系企讯网客服：
  
  电话：158-1425-6793

注册时间：2018-04-28
经营模式：生产型,
所在地区：广东省深圳市
联系人：苏元元女士
手　机：18665335907
手机扫描二维码
一键打电话给商家

电　话：0755-33164177
地　址：广东省深圳市深圳龙华民治

主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

企业官网

产品分类

酶联免疫ELISA试剂盒农残类检测试剂盒生化法试剂盒免疫学及试剂分子生物学试剂细胞生物学试剂检测试剂盒标准/常规溶液抗体抗原化学试剂实验室耗材细胞菌株代理进口专区动物疫病蛋白相关试剂虾类水产病原体检测系列

企业动态

Human HGF Quantikine ELISA Kit Summary

货号：DHG00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.25 hours or 4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Citrate Plasma (50 uL)

Sensitivity

40 pg/mL

Assay Range

125.0 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)

Specificity

Natural and recombinant human HGF

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human HGF Immunoassay is a 4.25 or 4.5 hour solid phase ELISA that is designed to measure HGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human pro-HGF and antibodies raised against the recombinant factor and has been shown to quantitate recombinant human HGF accurately. Results obtained using natural HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	339	999	3759	363	967	3790
Standard Deviation	23.7	56.2	154	25.8	81.2	205
CV%	7	5.6	4.1	7.1	8.4	5.4

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	425	991	3869	409	1058	4058
Standard Deviation	30.2	43.2	234	29.4	75.1	264
CV%	7.1	4.4	6	7.2	7.1	6.5

Recovery

The recovery of HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	92	86-101
Citrate Plasma (n=5)	105	99-114
EDTA Plasma (n=5)	100	85-111
Serum (n=5)	99	91-108

Linearity

To assess the linearity of the assay, five samples of each sample type were spiked with high concentrations of HGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human HGF ELISA Linearity