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Human CCL17/TARC Quantikine ELISA Kit
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
货号:DDN00
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 87 | 600 | 1107 | 98 | 616 | 1178 |
| Standard Deviation | 6.2 | 13.7 | 29.9 | 8.9 | 50.7 | 88.8 |
| CV% | 7.1 | 2.3 | 2.7 | 9.1 | 8.2 | 7.5 |
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 88 | 504 | 1001 | 89 | 540 | 1033 |
| Standard Deviation | 4.1 | 21.7 | 27.2 | 8.3 | 38.9 | 71.7 |
| CV% | 4.7 | 4.3 | 2.7 | 9.3 | 7.2 | 6.9 |
The recovery of TARC spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 88-104 |
| EDTA Plasma (n=5) | 102 | 86-111 |
| Heparin Plasma (n=5) | 98 | 86-107 |
| Serum (n=5) | 105 | 100-114 |