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Human Total IL-18/IL-1F4 Quantikine ELISA Kit
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
货号:DL180
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 175 | 301 | 636 | 197 | 381 | 739 |
| Standard Deviation | 5.49 | 8.06 | 24.6 | 27.4 | 46.7 | 81.1 |
| CV% | 3.1 | 2.7 | 3.9 | 13.9 | 12.3 | 11 |
Serum, EDTA Plasma, Heparin Plasma, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 126 | 251 | 495 | 126 | 248 | 494 |
| Standard Deviation | 3.85 | 6.33 | 14.6 | 10.9 | 19.7 | 41.6 |
| CV% | 3.1 | 2.5 | 2.9 | 8.7 | 7.9 | 8.4 |
The recovery of human Total IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 90-102 |
| EDTA Plasma (n=4) | 104 | 93-113 |
| Heparin Plasma (n=4) | 102 | 88-110 |
| Saliva (n=4) | 108 | 94-120 |
| Serum (n=4) | 100 | 96-105 |
| Urine (n=4) | 91 | 84-100 |