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Human PlGF Quantikine ELISA Kit
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
货号:DPG00
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 40.9 | 138 | 541 | 46.9 | 149 | 555 |
| Standard Deviation | 2.4 | 7.3 | 38.2 | 6.4 | 13.5 | 32.9 |
| CV% | 5.9 | 5.3 | 7.1 | 13.6 | 9.1 | 5.9 |
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 54.3 | 175 | 658 | 55 | 184 | 724 |
| Standard Deviation | 3.8 | 6.3 | 36.9 | 6.5 | 20.3 | 78.9 |
| CV% | 7 | 3.6 | 5.6 | 11.8 | 11 | 10.9 |
The recovery of PlGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 97 | 82-107 |
| Citrate Plasma (n=5) | 99 | 93-120 |
| EDTA Plasma (n=5) | 102 | 92-117 |
| Heparin Plasma (n=5) | 94 | 81-113 |
| Serum (n=5) | 96 | 88-118 |
| Urine (n=4) | 97 | 85-112 |