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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Human IL-6 R alpha Quantikine ELISA Kit Summary

  • Human IL-6 R alpha Quantikine ELISA Kit Summary

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Human IL-6 R alpha Quantikine ELISA Kit Summary
  • 产品描述：Human IL-6 R alpha Quantikine ELISA Kit Summary
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Human IL-6 R alpha Quantikine ELISA Kit Summary

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货号：DR600

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (50 uL)
Sensitivity
15.1 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human IL-6 sR
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-6 sR Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-6 sR in cell culture supernates, serum, plasma, and urine. It contains recombinant human IL-6 sR produced in Sf 21 cells and antibodies raised against this protein. This kit has been shown to accurately quantitate the recombinant soluble IL-6 R. Results obtained using natural IL-6 sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-6 sR. Since the measurement of human IL-6 sR by this immunoassay is insensitive to added recombinant human IL-6, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples ( i.e. the total amount of free receptor plus the total amount of receptor bound to IL-6).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 134 644 1669 130 645 1593
Standard Deviation 11.5 16.8 38.3 8.3 26.9 75.4
CV% 8.6 2.6 2.3 6.4 4.2 4.7

Recovery

The recovery of IL-6 sR spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 85-97
Citrate Plasma (n=4) 93 86-102
EDTA Plasma (n=4) 93 76-107
Heparin Plasma (n=4) 101 87-118
Serum (n=4) 93 86-110
Urine (n=4) 86 64-98

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of natural or recombinant human IL-6 sR were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the as
Human IL-6 R alpha ELISA Linearity
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  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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