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Human CCL20/MIP-3 alpha Quantikine ELISA Kit
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
货号:DM3A00
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 66.2 | 180 | 372 | 68.7 | 186 | 357 |
| Standard Deviation | 1.84 | 3.83 | 15.1 | 4.51 | 13 | 28.8 |
| CV% | 2.8 | 2.1 | 4.1 | 6.6 | 6.9 | 8.1 |
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 55.9 | 161 | 323 | 72.5 | 200 | 386 |
| Standard Deviation | 1.62 | 4.28 | 9.1 | 6.05 | 17.9 | 35.3 |
| CV% | 2.9 | 2.7 | 2.8 | 8.3 | 8.9 | 9.1 |
The recovery of MIP-3 alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 101 | 88-115 |
| EDTA Plasma (n=5) | 104 | 97-115 |
| Heparin Plasma (n=5) | 104 | 98-112 |
| Serum (n=5) | 103 | 94-112 |