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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Human sE-Selectin/CD62E Quantikine ELISA Kit

  • Human sE-Selectin/CD62E Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Human sE-Selectin/CD62E Quantikine ELISA Kit
  • 产品描述：Human sE-Selectin/CD62E Quantikine ELISA Kit
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Human sE-Selectin/CD62E Quantikine ELISA Kit Summary

Catalog              Availability Size / Price

DSLE00              1 Kit / Please Inquire
SSLE00              1 Pack (6 Plates) / Please Inquire

PDSLE00            1 Pack (50 Plates) / Please Inquire

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Sensitivity
0.027 ng/mL
Assay Range
0.1 - 8 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human sE-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human sE-Selectin immunoassay is a 4.5 hour solid phase ELISA designed to measure sE-Selectin in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sE-Selectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sE-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human sE-Selectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1.6 2.9 4.7 1.5 2.9 4.6
Standard Deviation 0.11 0.17 0.24 0.11 0.25 0.35
CV% 6.6 5.7 5.2 7.3 8.7 7.7

Recovery

The recovery of sE-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-110
Citrate Plasma (n=4) 96 85-107
Heparin Plasma (n=4) 94 85-107
Serum (n=4) 96 85-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of sE-Selectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human E-Selectin/CD62E ELISA Linearity

深圳子科生物科技有限公司
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  • 手　机：18665335907
  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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