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Human MIF Quantikine ELISA Kit
Catalog Availability
DMF00B 1 Kit / Please Inquire
SMF00B 1 Pack (6 Plates) / Please Inquire
PDMF00B 1 Pack (50 Plates) / Please Inquire
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.16 | 3.56 | 7.27 | 1.03 | 3.2 | 6.46 |
| Standard Deviation | 0.07 | 0.18 | 0.33 | 0.1 | 0.27 | 0.61 |
| CV% | 6 | 5.1 | 4.5 | 9.7 | 8.4 | 9.4 |
The recovery of MIF spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 86-104 |
| Platelet-poor EDTA Plasma (n=5) | 95 | 80-108 |
| Platelet-poor Heparin Plasma (n=5) | 96 | 86-108 |
| Serum (n=4) | 99 | 94-104 |