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Human u-Plasminogen Activator/Urokinase Quantikine ELISA
货号:DUPA00 规格:96T
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 230 | 629 | 1219 | 214 | 614 | 1188 |
| Standard Deviation | 4.8 | 8.9 | 29.3 | 15.1 | 39.8 | 82 |
| CV% | 2.1 | 1.4 | 2.4 | 7.1 | 6.5 | 6.9 |
The recovery of uPA spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 102 | 93-115 |
| Cell Lysates (n=4) | 97 | 81-116 |
| EDTA Plasma (n=4) | 87 | 83-94 |
| Heparin Plasma (n=4) | 89 | 81-110 |
| Serum (n=4) | 87 | 81-93 |
| Urine (n=4) | 98 | 81-119 |