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Mouse/Rat PDGF-BB Quantikine ELISA Kit
Mouse/Rat PDGF-BB Quantikine ELISA Kit Summary
货号：MBB00 规格：96T
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Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 24 | 24 |
| Mean | 49.7 | 252 | 823 | 52.1 | 246 | 811 |
| Standard Deviation | 4.5 | 18.7 | 36.7 | 5.3 | 21.1 | 40.4 |
| CV% | 9.1 | 7.4 | 4.5 | 10.2 | 8.6 | 5 |
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 22 | 22 | 23 |
| Mean | 61.1 | 298 | 1019 | 69.5 | 305 | 1048 |
| Standard Deviation | 5.6 | 24.6 | 40.3 | 6.6 | 26.3 | 98.5 |
| CV% | 9.2 | 8.3 | 4 | 9.5 | 8.6 | 9.4 |
The recovery of PDGF-BB spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=8) | 100 | 89-118 |
| Mouse Heparin Plasma (n=5) | 96 | 85-113 |
| Rat Cell Culture Supernates (n=8) | 102 | 94-114 |
| Rat EDTA Plasma (n=5) | 102 | 86-111 |
| Rat Heparin Plasma (n=5) | 102 | 85-111 |
| Rat Serum (n=5) | 100 | 89-113 |
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