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Human LIF Quantikine ELISA Kit
Human LIF Quantikine ELISA Kit Summary
货号:DLF00
规格:96T
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 341 | 875 | 1358 | 81.6 | 472 | 910 |
| Standard Deviation | 12.3 | 34.5 | 42.5 | 4.4 | 17.7 | 35.2 |
| CV% | 3.6 | 3.9 | 3.1 | 5.4 | 3.8 | 3.9 |
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 314 | 868 | 1347 | 87.4 | 524 | 1017 |
| Standard Deviation | 7.7 | 24.3 | 23.8 | 6.2 | 34.9 | 46.5 |
| CV% | 2.5 | 2.8 | 1.8 | 7.1 | 6.7 | 4.6 |
The recovery of LIF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 91-106 |
| EDTA Plasma (n=2) | 89 | 84-97 |
| Heparin Plasma (n=8) | 92 | 84-100 |
| Serum (n=11) | 102 | 91-110 |