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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Mouse C-Reactive Protein/CRP Quantikine ELISA Kit

  • Mouse C-Reactive Protein/CRP Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
  • 产品描述：Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
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Mouse C-Reactive Protein/CRP Quantikine ELISA Kit Summary

货号：MCRP00  规格：96T


Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.015 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse CRP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse C-Reactive Protein/CRP immunoassay is a 4.5 hour solid-phase ELISA designed to measure CRP in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse CRP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse CRP. Results obtained using natural mouse CRP showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse CRP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.453 1.31 3.78 0.48 1.33 3.76
Standard Deviation 0.035 0.083 0.089 0.052 0.087 0.314
CV% 7.7 6.3 2.4 10.8 6.5 8.4

Recovery

The recovery of mouse CRP spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 82-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse CRP in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse C-Reactive Protein/CRP ELISA Linearity


MCRP00


深圳子科生物科技有限公司
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  • 手　机：18665335907
  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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