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Human LIGHT/TNFSF14 Quantikine ELISA Kit
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
货号:DLIT00
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 350 | 712 | 1388 | 387 | 754 | 1469 |
| Standard Deviation | 12 | 31.7 | 63.3 | 19.6 | 31.8 | 63.4 |
| CV% | 3.4 | 4.5 | 4.6 | 5.1 | 4.2 | 4.3 |
Cell Culture Supernates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 308 | 626 | 1181 | 318 | 616 | 1177 |
| Standard Deviation | 11.7 | 25.3 | 34.6 | 27 | 44.2 | 81.8 |
| CV% | 3.8 | 4 | 2.9 | 8.5 | 7.2 | 6.9 |
The recovery of LIGHT spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 100-113 |
| EDTA Plasma (n=5) | 106 | 91-114 |
| Heparin Plasma (n=5) | 101 | 89-115 |
| Serum (n=5) | 102 | 85-114 |