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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit

  • Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Human Butyrylcholinesterase/BCHE Quantikine ELISA
  • 产品描述：Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit
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Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit Summary

货号：DBCHE0  规格：96T

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
0.06 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human BCHE.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Butyrylcholinesterase/BCHE Immunoassay is a 4.5 hour solid phase ELISA designed to measure BCHE levels in cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human BCHE and antibodies raised against the recombinant protein. Results obtained for naturally occurring human BCHE showed linear curves that were parallel to the standard curves obtained using the Quantikine Human BCHE Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human BCHE.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.25 2.41 5.01 1.26 2.51 5.11
Standard Deviation 0.031 0.044 0.154 0.063 0.114 0.177
CV% 2.5 1.8 3.1 3.1 4.5 3.5

Recovery

The recovery of human BCHE spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 94-105
Cell Lysis Buffer 1 (n=2) 99 96-102
Urine (n=4) 97 93-102

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human BCHE were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Butyrylcholinesterase/BCHE ELISA Linearity

深圳子科生物科技有限公司
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  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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