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深圳子科生物科技有限公司 http://www.114my.cn/shopdetail/157512/

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  • 注册时间:2018-04-28
  • 经营模式:生产型,
  • 所在地区:广东省 深圳市
  • 联系人:苏元元女士
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  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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  • Canine CCL2/MCP-1 Quantikine ELISA Kit
  • Canine CCL2/MCP-1 Quantikine ELISA Kit

  • 发布时间:2021-11-01 11:41:37
  • 关键词:Canine CCL2/MCP-1 Quantikine ELISA Kit
  • 产品描述:Canine CCL2/MCP-1 Quantikine ELISA Kit
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Canine CCL2/MCP-1 Quantikine ELISA Kit

Catalog # Availability Size / Price
CACP00 Contact your distributor for availability. 1 Kit / Please Inquire

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
10.8 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant canine MCP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Canine MCP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine MCP-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine MCP-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine MCP-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 43 43 43
Mean 37.1 144 632 42.7 133 655
Standard Deviation 2.2 5.8 22 5.4 7.5 30
CV% 5.9 4 3.5 12.6 5.6 4.6

Recovery

The recovery of canine MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 112 106-121
EDTA Plasma (n=4) 110 102-116
Heparin Plasma (n=4) 103 96-112
Serum (n=4) 93 84-102

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of canine MCP-1 in each matrix were diluted with Calibrator Diluent and assayed.
Canine CCL2/JE/MCP-1 ELISA Linearity

深圳子科生物科技有限公司
  • 联系人:苏元元女士
  • 手 机:18665335907
  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

深圳子科生物科技有限公司 地址:广东省 深圳市 深圳龙华民治 技术支持:商铺管理入口 | 产品索引 | 免责声明