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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Human MIA Quantikine ELISA Kit

  • Human MIA Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Human MIA Quantikine ELISA Kit
  • 产品描述：Human MIA Quantikine ELISA Kit
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深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Human MIA Quantikine ELISA Kit

Catalog # Availability Size / Price
DMIA00 Contact your distributor for availability. 1 Kit / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (50 uL)
Sensitivity
8.46 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human MIA
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIA Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MIA in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant Human MIA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MIA. Results obtained using natural human MIA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIA.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 158 315 622 150 308 627
Standard Deviation 5.5 10.9 17.8 7.02 15.7 37.8
CV% 3.5 3.5 2.9 4.7 5.1 6

Recovery

The recovery of MIA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 101-107
EDTA Plasma (n=4) 98 85-115
Heparin Plasma (n=4) 99 85-113
Saliva (n=4) 102 86-110
Serum (n=4) 101 91-118

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIA were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Human MIA ELISA Linearity

深圳子科生物科技有限公司
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  • 手　机：18665335907
  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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