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CM0004D营养琼脂片剂

A general purpose medium which may be enriched with up to 10% blood or other biological fluid.

* Adjusted as required to meet performance standards.

Directions
Powder: Suspend 28g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Tablets: Add one tablet to 5ml of distilled water and soak for 5 minutes. Sterilise by autoclaving at 121°C for 15 minutes.

Description
Nutrient Agar is a basic culture medium used to subculture organisms for maintenance purposes or to check the purity of subcultures from isolation plates prior to biochemical or serological tests.

In semi-solid form, agar slopes or agar butts, the medium is used to maintain control organisms¹.

Nutrient Agar is suitable for teaching and demonstration purposes. It contains a concentration of 1.5% agar to permit the addition of up to 10% blood or other biological fluid, as required. The medium, without additions, may be used for the cultivation of organisms which are not exacting in their nutritional requirements.

For a medium which is richer in nutrients, see Blood Agar Base No.2 CM0271.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel

Quality control

* This organism is available as a Culti-Loop®

Reference
1. Lapage S. P., Shelton J. E. and Mitchell T. G. (1970)` in Methods in Microbiology’ Eds. Norris J. R. and Ribbons D. W. Vol.3A. Academic Press. London. p.116.