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重组牛肠激酶轻链，大肠杆菌表达
重组牛肠激酶轻链，大肠杆菌表达
货号：zk2198
英文名： Recombinant Bovine Enterokinase Light Chain
别名： EK, Bovine, E.coli-derived
相关类别： 酶
储存冷冻：（-20℃）
分子量： Approximately 28 kDa, a single non-glycosylated polypeptide chain containing 235 amino acids.
规格：100 IU
Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfide-linked 82–140 k Da heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 k Da light chain which contains the catalytic subunit. Additionally, both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue. rEK was report to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications.
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
产品概述：
使用说明
技术规格
Physical Appearance
Sterile liquid.
Source
Escherichia coli.
Formulation
50 mM Tris-HCl, pH 8.0, 0.5 M NaCl and 50 % glycerol.
Endotoxin
Less than 1 EU/μg of rBoEKL as determined by LAL method.
Unit Definition
One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95 % completion at 25 °C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.
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