手机网站
企讯网推荐企业
手机商铺
手机商铺
扫描进入手机商铺
深圳子科生物科技有限公司 http://www.114my.cn/shopdetail/157512/

主营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

商铺首页 产品展示 企业简介 企业动态 招聘中心 在线留言 联系我们
已认证企业
深圳子科生物科技有限公司
  • 会8年
  • 说明
    • 会 会员认证：网站通过企讯网认证，真实合法，放心访问；
    • 官网认证：网站通过工信部备案，真实合法，放心访问；
    • 工商认证：企业通过工商注册，营业执照真实有效；
    • 加V认证：企业通过百度信誉认证，百度优先赔付；
    • 诚信通认证：企业在阿里巴巴诚信通有资料，已经开通阿里巴巴旺铺；
    • 微店认证：企业开通朝阳微商城，可以通过移动网络购买商品。
    • 认证或商铺装修请联系企讯网客服： 点击这里给我发消息
      电 话：158-1425-6793
  • 注册时间：2018-04-28
  • 经营模式：生产型,
  • 所在地区：广东省 深圳市
  • 联系人：苏元元女士
  • 手　机：18665335907
    商铺信息手机扫描二维码
    一键打电话给商家
  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

产品分类

企业动态

最新产品


  • 咨询

  • 商铺
    手机商铺
    手机扫描二维码
    进入手机商铺

  • 一键联系商家
    商铺信息
    • 联系人：苏元元女士
    • 职　务：总经理
    • 手　机：18665335907
    • 电　话：0755-33164177
    手机扫描二维码一键打电话给商家
手机商铺

手机查看产品信息

首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit

  • Human IL-1 beta/IL-1F2 Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
  • 产品描述：Human IL-1 beta/IL-1F2 Quantikine ELISA Kit
  • 留言询价
  • 详细信息
  • 联系我们

Human IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary

Catalog        Size / Price
DLB50           1 Kit / Please Inquire
SLB50           1 Pack (6 Plates) / Please Inquire

PDLB50         1 Pack (50 Plates) / Please Inquire

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
Sensitivity
1 pg/mL
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-1 beta Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-1 beta accurately. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.

Precision

Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 1 2 3
n 20 20 20 20 20 20 20
Mean 18.9 30.2 122 181 29.8 118 193
Standard Deviation 1.6 1 5.4 5 2.5 5 8
CV% 8.5 3.3 4.4 2.8 8.4 4.2 4.1

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 1 2 3
n 20 20 20 20 20 20 20
Mean 12.6 30.4 69.2 192 30.8 120 194
Standard Deviation 0.3 1 1.6 6.5 2.2 4.1 7.9
CV% 2.4 3.3 2.3 3.4 7.1 3.4 4.1

Recovery

The recovery of IL-1 beta spiked to different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 80-111
Citrate Plasma (n=10) 93 83-110
EDTA Plasma (n=10) 86 81-100
Heparin Plasma (n=10) 82 76-100
Serum (n=10) 95 87-110


深圳子科生物科技有限公司
  • 联系人：苏元元女士
  • 手　机：18665335907
  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

深圳子科生物科技有限公司 地址：广东省 深圳市 深圳龙华民治 技术支持：商铺管理入口 | 产品索引 | 免责声明

该网上商铺由企讯网提供  资料由企业自行管理  企讯网投诉邮箱：tousu@114my.com