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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Human ErbB2/Her2 Quantikine ELISA Kit

  • Human ErbB2/Her2 Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Human ErbB2/Her2 Quantikine ELISA Kit
  • 产品描述：Human ErbB2/Her2 Quantikine ELISA Kit
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Human ErbB2/Her2 Quantikine ELISA Kit Summary

货号：DHER20 规格：96T

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
14.8 pg/mL
Assay Range
78.1 - 5,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant human ErbB2.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human ErbB2/Her2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ErbB2 in cell culture supernates, cell lysates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human ErbB2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ErbB2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ErbB2.

Precision

Intra-Assay Precision (Precision within an assay) Intra-assay Precision (Precision within an assay)<br>Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Inter-assay Precision (Precision between assays)<br>Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 471 1379 2681 489 1419 2667
Standard Deviation 6.71 16.3 31.1 18.7 56.4 93.5
CV% 1.4 1.2 1.2 3.8 4 3.5

Recovery

The recovery of human ErbB2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 90-120
EDTA Plasma (n=4) 99 89-118
Heparin Plasma (n=4) 104 92-119
Serum (n=4) 99 91-115
Urine (n=4) 101 94-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ErbB2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human ErbB2/Her2 ELISA Linearity

深圳子科生物科技有限公司
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  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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