Rat TNF-alpha Quantikine ELISA Kit【行情报价价格评测】-企讯网

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Rat TNF-alpha Quantikine ELISA Kit Summary

货号：RTA00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Specificity

Natural and recombinant rat TNF-alpha

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat TNF-alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat TNF-alpha accurately. Results obtained using natural rat TNF-alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat TNF-alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	65	232	593	63	246	656
Standard Deviation	3.3	5.1	12.5	6.1	23.6	57.6
CV%	5.1	2.2	2.1	9.7	9.6	8.8

Recovery

The recovery of rat TNF-alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=6)	101	93-119
EDTA Plasma (n=5)	96	88-104
Heparin Plasma (n=4)	80	73-84
Serum (n=8)	93	83-105

Linearity

To assess the linearity of the assay, samples spiked with various concentrations of rat TNF-alpha were diluted with Calibrator Diluent and then assayed. Results from typical sample dilutions are shown.

Rat TNF-alpha ELISA Linearity