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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Mouse LIX Quantikine ELISA Kit

  • Mouse LIX Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Mouse LIX Quantikine ELISA Kit
  • 产品描述：Mouse LIX Quantikine ELISA Kit
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Mouse LIX Quantikine ELISA Kit Summary

货号：MX000  规格：96T

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (13 uL), Platelet-poor Heparin Plasma (13 uL), Urine (13 uL)
Sensitivity
1.74 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse LIX
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse CXCL5/LIX Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIX levels in cell culture supernates, tissue lysates, serum, platelet-poor plasma, and urine. It contains E. coli-expressed recombinant mouse LIX and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse LIX accurately. Results obtained using natural mouse LIX showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse LIX.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 42.7 133 407 41.8 120 393
Standard Deviation 1.72 5.93 10.9 2.81 4.64 20.3
CV% 4 4.5 2.7 6.7 3.9 5.2

Recovery

The recovery of mouse LIX spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 114 105-120
Platelet-poor EDTA Plasma (n=4) 100 92-109
Platelet-poor Heparin Plasma (n=4) 91 85-99
Serum (n=4) 87 82-94
Tissue Homogenates (n=4) 100 87-113
Urine (n=4) 91 84-96

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse LIX were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Mouse LIX ELISA Linearity

深圳子科生物科技有限公司
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  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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