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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Mouse IL-6 R alpha Quantikine ELISA Kit

  • Mouse IL-6 R alpha Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Mouse IL-6 R alpha Quantikine ELISA Kit
  • 产品描述：Mouse IL-6 R alpha Quantikine ELISA Kit
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Mouse IL-6 R alpha Quantikine ELISA Kit

Catalog # Availability Size / Price
MR600 Contact your distributor for availability. 1 Kit / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.07 ng/mL
Assay Range
0.2 - 12 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-6 R alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-6 R alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-6 R alpha levels in cell culture supernates, serum, and heparin plasma. It contains NS0-expressed recombinant mouse IL-6 R alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-6 R alpha accurately. Results obtained using natural mouse IL-6 R alpha showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6 R alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.456 1.07 2.94 0.474 1.08 2.82
Standard Deviation 0.036 0.039 0.148 0.06 0.08 0.2
CV% 7.9 3.6 5 12.7 7.4 7.1

Recovery

The recovery of mouse IL-6 R alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 90-110
Heparin Plasma (n=4) 99 92-106
Serum (n=4) 102 93-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-6 R alpha were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse IL-6 R alpha ELISA Linearity

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  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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