Human CXCL12/SDF-1 alpha Quantikine ELISA Kit【行情报价价格评测】-企讯网

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Human CXCL12/SDF-1 alpha Quantikine ELISA Kit Summary

货号：DSA00

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 uL), EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)

Sensitivity

47 pg/mL

Assay Range

156.0 - 10,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma)

Specificity

Natural and recombinant SDF-1 alpha

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma. It contains E. coli-expressed recombinant human SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SDF-1 alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	491	1700	3420	544	1600	3150
Standard Deviation	19	58	115	73	150	257
CV%	3.9	3.4	3.4	13.4	9.4	8.2

Recovery

The recovery of SDF-1 alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	95-108
Platelet-poor EDTA Plasma (n=5)	97	90-107
Platelet-poor Heparin Plasma (n=5)	97	90-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CXCL12/SDF-1 alpha ELISA Linearity