Hyaluronan Quantikine ELISA Kit【行情报价价格评测】-企讯网

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Hyaluronan Quantikine ELISA Kit Summary

货号：DHYAL0

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.2 ng/mL

Assay Range

0.6 - 40 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Measures greater than or equal to 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine samples

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Hyaluronan Immunoassay is a 4.5 hour solid-phase ELISA designed to measure at least 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine cell culture supernates, tissue homogenates, serum, and plasma samples. It contains Streptococcus pyogenes-derived Hyaluronan and NS0-expressed human Aggrecan. This immunoassay has been shown to accurately quantitate Hyaluronan.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.53	4.81	14.3	1.68	4.68	14.98
Standard Deviation	0.11	0.26	0.52	0.08	0.23	0.72
CV%	7.2	5.4	3.6	4.8	4.9	4.8

Recovery

The recovery of Hyaluronan spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	104	96-116
EDTA Plasma (n=10)	97	83-106
Heparin Plasma (n=10)	99	80-124
Serum (n=10)	98	81-119

Linearity

To assess the linearity of the assay, samples containing high concentrations of Hyaluronan were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.