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Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
货号:DLA50
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 28.6 | 125 | 185 | 27.9 | 123 | 187 |
| Standard Deviation | 0.4 | 1.4 | 4 | 1.2 | 4.7 | 6.3 |
| CV% | 1.4 | 1.1 | 2.2 | 4.3 | 3.8 | 3.4 |
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 26.9 | 122 | 191 | 20.5 | 60 | 121 |
| Standard Deviation | 0.4 | 4.6 | 6.6 | 1.7 | 3.1 | 5.2 |
| CV% | 1.5 | 3.8 | 3.5 | 8.3 | 5.2 | 4.3 |
The recovery of IL-1 alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 91-104 |
| Citrate Plasma (n=6) | 97 | 71-119 |
| EDTA Plasma (n=6) | 104 | 84-121 |
| Heparin Plasma (n=6) | 104 | 72-126 |
| Serum (n=6) | 107 | 74-127 |