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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Human MFG-E8 Quantikine ELISA Kit

  • Human MFG-E8 Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Human MFG-E8 Quantikine ELISA Kit
  • 产品描述：Human MFG-E8 Quantikine ELISA Kit
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Human MFG-E8 Quantikine ELISA Kit Summary

Catalog # DFGE80 

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Saliva (50 uL), Urine (50 uL), Human Milk (10 uL)
Sensitivity
13.4 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human MFG-E8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MFG-E8 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MFG-E8. Results obtained using natural human MFG-E8 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MFG-E8.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) <div>Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components. </div>

Cell Culture Supernates, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 680 1181 2103 692 1262 2206
Standard Deviation 30.5 38.9 84.7 57.6 88 125
CV% 4.5 3.3 4 8.3 7 5.7

Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 645 1143 2168 749 1326 2339
Standard Deviation 39.5 46.6 81.1 57.4 78 117
CV% 6.1 4.1 3.7 7.7 5.9 5

Recovery

The recovery of human MFG-E8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 100-110
EDTA Plasma (n=4) 96 89-102
Heparin Plasma (n=4) 96 89-102
Serum (n=4) 101 92-110

Linearity

To assess the linearity of the assay, samples containing high concentrations of MFG-E8 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Human MFG-E8 ELISA Linearity

深圳子科生物科技有限公司
  • 联系人：苏元元女士
  • 手　机：18665335907
  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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