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Human IL-17 Quantikine ELISA Kit Summary
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
货号:D1700
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 131 | 736 | 1480 | 125 | 408 | 794 |
| Standard Deviation | 5.8 | 34.6 | 61.2 | 9.8 | 34.3 | 55.6 |
| CV% | 4.4 | 4.7 | 4.1 | 7.8 | 8.4 | 7 |
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 107 | 604 | 1217 | 76.6 | 272 | 549 |
| Standard Deviation | 7.8 | 25 | 63.3 | 6.5 | 23.4 | 43.9 |
| CV% | 7.3 | 4.1 | 5.2 | 8.5 | 8.6 | 8 |
The recovery of human IL-17 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 97 | 88-112 |
| Citrate Plasma (n=5) | 105 | 92-116 |
| EDTA Plasma (n=5) | 101 | 90-110 |
| Heparin Plasma (n=5) | 101 | 93-109 |
| Serum (n=5) | 98 | 90-105 |