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Human Flt-3 Ligand Quantikine ELISA Kit Summary
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
货号:DFK00
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 65.7 | 214 | 421 | 72.8 | 224 | 429 |
| Standard Deviation | 1.2 | 4.1 | 9.3 | 6.7 | 13.8 | 30.5 |
| CV% | 1.8 | 1.9 | 2.2 | 9.2 | 6.2 | 7.1 |
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 69.5 | 230 | 453 | 78.2 | 241 | 462 |
| Standard Deviation | 1.9 | 5.2 | 6.2 | 8.7 | 14.9 | 33.1 |
| CV% | 2.7 | 2.3 | 1.4 | 11.1 | 6.2 | 7.2 |
The recovery of Flt-3 Ligand spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 102 | 92-118 |
| Citrate Plasma (n=5) | 101 | 94-110 |
| EDTA Plasma (n=5) | 101 | 90-119 |
| Heparin Plasma (n=5) | 100 | 88-112 |
| Serum (n=5) | 100 | 91-117 |