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  • 注册时间:2018-04-28
  • 经营模式:生产型,
  • 所在地区:广东省 深圳市
  • 联系人:苏元元女士
  • 手 机:18665335907
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  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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  • Human TGF-beta 2 Quantikine ELISA Kit
  • Human TGF-beta 2 Quantikine ELISA Kit

  • 发布时间:2021-11-01 11:41:37
  • 关键词:Human TGF-beta 2 Quantikine ELISA Kit
  • 产品描述:Human TGF-beta 2 Quantikine ELISA Kit
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Human TGF-beta 2 Quantikine ELISA Kit Summary

深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!

货号:DB250

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (125 uL), Serum (125 uL), EDTA Plasma (125 uL), Heparin Plasma (125 uL), Citrate Plasma (125 uL)
Sensitivity
7 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human TGF-beta 2 and natural porcine TGF-beta 2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human TGF-beta 2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure activated TGF-beta 2 in cell culture supernates, serum, and plasma. The Quantikine Human TGF-beta 2 Immunoassay contains CHO cell-derived recombinant human TGF-beta 2 and antibodies raised against the recombinant protein. The assay has been shown to quantitate the recombinant factor accurately. Results obtained using human TGF-beta 2 showed linear curves that were parallel to the standard curves obtained using the recombinant TGF-beta 2 kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TGF-beta 2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 194 509 1044 196 500 1015
Standard Deviation 13.8 27 28.3 9.8 21.5 43.6
CV% 7.1 5.3 2.7 5 4.3 4.3

Recovery

To assess recovery of the assay, samples of various matrices were spiked with three concentrations of TGF-beta 2, activated, and measured in the assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 101 89-113
Citrate Plasma (n=5) 107 93-116
EDTA Plasma (n=5) 101 95-111
Heparin Plasma (n=5) 102 89-113
Serum (n=5) 86 73-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of TGF-beta 2, activated and then serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TGF-beta 2 ELISA Linearity
深圳子科生物科技有限公司
  • 联系人:苏元元女士
  • 手 机:18665335907
  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

深圳子科生物科技有限公司 地址:广东省 深圳市 深圳龙华民治 技术支持:商铺管理入口 | 产品索引 | 免责声明