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深圳子科生物科技有限公司 http://www.114my.cn/shopdetail/157512/

主营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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  • 注册时间:2018-04-28
  • 经营模式:生产型,
  • 所在地区:广东省 深圳市
  • 联系人:苏元元女士
  • 手 机:18665335907
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  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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  • Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
  • Human Pentraxin 3/TSG-14 Quantikine ELISA Kit

  • 发布时间:2021-11-01 11:41:37
  • 关键词:Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
  • 产品描述:Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
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Human Pentraxin 3/TSG-14 Quantikine ELISA Kit Summary

货号:DPTX30B  规格:96T

Assay Length
5.0 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (20 uL), EDTA Plasma (20 uL), Saliva (20 uL)
Sensitivity
0.026 ng/mL
Assay Range
0.3 - 20 ng/mL (Serum-free Cell Culture Supern, EDTA Plasma, Saliva)
Specificity
Natural and recombinant human Pentraxin 3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Pentraxin 3/TSG-14 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human Pentraxin 3 in serum-free cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human Pentraxin 3 and antibodies raised against the recombinant protein. Natural human Pentraxin 3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human 
Pentraxin 3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum-free Cell Culture Supernates, EDTA Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 2.12 6.03 11.6 2.05 5.86 11.4
Standard Deviation 0.038 0.158 0.14 0.141 0.383 0.5
CV% 1.8 2.6 1.2 6.9 6.5 4.4

Recovery

The recovery of human Pentraxin 3 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 99 85-108
Saliva (n=4) 89 79-99
Serum-free Cell Culture Media (n=2) 109 100-120

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Pentraxin 3 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Pentraxin 3/TSG-14 ELISA Linearity
深圳子科生物科技有限公司
  • 联系人:苏元元女士
  • 手 机:18665335907
  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

深圳子科生物科技有限公司 地址:广东省 深圳市 深圳龙华民治 技术支持:商铺管理入口 | 产品索引 | 免责声明