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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Human Coagulation Factor III/Tissue Factor Quantikine ELISA

  • Human Coagulation Factor III/Tissue Factor Quantikine ELISA

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Human Coagulation Factor III/Tissue Factor Quantik
  • 产品描述：Human Coagulation Factor III/Tissue Factor Quantikine ELISA
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Human Coagulation Factor III/Tissue Factor Quantikine ELISA Summary


货号：DCF300

深圳子科生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL), Urine (50 uL)
Sensitivity
2.05 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human Coagulation Factor III
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.


Product Summary

The Quantikine Human Coagulation Factor III/Tissue Factor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Coagulation Factor III in cell culture supernates, cell lysates, plasma, and urine. It contains NS0-expressed recombinant human Coagulation Factor III and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Coagulation Factor III showed linear curves that were p arallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Coagulation Factor III.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 33.4 65.4 148 71 144 301
Standard Deviation 0.78 1.81 5 4.5 7.8 17.1
CV% 2.3 2.8 3.4 6.3 5.4 5.7

Recovery

The recovery of Coagulation Factor III spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 95-110
Citrate Plasma (n=4) 92 86-102
EDTA Plasma (n=4) 91 86-97
Heparin Plasma (n=4) 95 88-109
Urine (n=4) 102 96-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Coagulation Factor III were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Coagulation Factor III/Tissue Factor ELISA Linearity


深圳子科生物科技有限公司
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  • 手　机：18665335907
  • 电　话：0755-33164177
  • 地　址：广东省深圳市深圳龙华民治
  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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