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Human KGF/FGF-7 Quantikine ELISA Kit
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货号：DKG00
规格：96T
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 97.9 | 298 | 1229 | 95.5 | 294 | 1221 |
| Standard Deviation | 3.3 | 8.8 | 42.8 | 7.4 | 15.6 | 63.5 |
| CV% | 3.4 | 3 | 3.5 | 7.7 | 5.3 | 5.2 |
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 82.4 | 248 | 1031 | 86.5 | 266 | 1108 |
| Standard Deviation | 3.2 | 8.8 | 55.2 | 4.8 | 11.4 | 62 |
| CV% | 3.9 | 3.5 | 5.4 | 5.5 | 4.3 | 5.6 |
The recovery of KGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 102 | 95-105 |
| Citrate Plasma (n=5) | 91 | 86-98 |
| EDTA Plasma (n=5) | 96 | 85-104 |
| Heparin Plasma (n=5) | 90 | 85-99 |
| Serum (n=5) | 93 | 85-100 |
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