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Human Total MMP-8 Quantikine ELISA Kit
Human Total MMP-8 Quantikine ELISA Kit Summary
货号:DMP800B
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Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.764 | 2.38 | 4.55 | 0.836 | 2.41 | 5.07 |
| Standard Deviation | 0.049 | 0.07 | 0.299 | 0.055 | 0.171 | 0.27 |
| CV% | 6.4 | 2.9 | 6.6 | 6.6 | 7.1 | 5.3 |
The recovery of human Total MMP-8 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 93-103 |
| EDTA Plasma (n=4) | 92 | 86-99 |
| Extraction Buffer (1X) (n=2) | 84 | 83-85 |
| Heparin Plasma (n=4) | 92 | 86-99 |
| Platelet-poor EDTA Plasma (n=4) | 85 | 79-91 |
| Platelet-poor Heparin Plasma (n=4) | 84 | 78-89 |
| Saliva (n=4) | 96 | 86-111 |
| Serum (n=4) | 101 | 93-109 |