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  • 注册时间:2018-04-28
  • 经营模式:生产型,
  • 所在地区:广东省 深圳市
  • 联系人:苏元元女士
  • 手 机:18665335907
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  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
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  • Human Total MMP-8 Quantikine ELISA Kit
  • Human Total MMP-8 Quantikine ELISA Kit

  • 发布时间:2021-11-01 11:41:37
  • 关键词:Human Total MMP-8 Quantikine ELISA Kit
  • 产品描述:Human Total MMP-8 Quantikine ELISA Kit
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Human Total MMP-8 Quantikine ELISA Kit Summary

货号:DMP800B

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Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (25 uL), Platelet-poor EDTA Plasma (50 uL), Heparin Plasma (25 uL), Platelet-poor Heparin Plasma (50 uL), Saliva (10 uL)
Sensitivity
0.058 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva)
Specificity
Natural and recombinant human MMP-8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.


Product Summary

The Quantikine® Human Total MMP-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total MMP-8 (pro- and active MMP-8) in cell culture supernates, tissue lysates, serum, plasma, platelet-poor plasma, and saliva. It contains NS0-expressed recombinant human MMP-8, and antibodies raised against the recombinant protein. Natural human MMP-8 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MMP-8.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 0.764 2.38 4.55 0.836 2.41 5.07
Standard Deviation 0.049 0.07 0.299 0.055 0.171 0.27
CV% 6.4 2.9 6.6 6.6 7.1 5.3

Recovery

The recovery of human Total MMP-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 93-103
EDTA Plasma (n=4) 92 86-99
Extraction Buffer (1X) (n=2) 84 83-85
Heparin Plasma (n=4) 92 86-99
Platelet-poor EDTA Plasma (n=4) 85 79-91
Platelet-poor Heparin Plasma (n=4) 84 78-89
Saliva (n=4) 96 86-111
Serum (n=4) 101 93-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Total MMP-8 in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Total MMP-8 ELISA Linearity


深圳子科生物科技有限公司
  • 联系人:苏元元女士
  • 手 机:18665335907
  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

深圳子科生物科技有限公司 地址:广东省 深圳市 深圳龙华民治 技术支持:商铺管理入口 | 产品索引 | 免责声明