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主营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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  • 注册时间:2018-04-28
  • 经营模式:生产型,
  • 所在地区:广东省 深圳市
  • 联系人:苏元元女士
  • 手 机:18665335907
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  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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  • Human IGF-II Quantikine ELISA Kit
  • Human IGF-II Quantikine ELISA Kit

  • 发布时间:2021-11-01 11:41:37
  • 关键词:Human IGF-II Quantikine ELISA Kit
  • 产品描述:Human IGF-II Quantikine ELISA Kit
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Human IGF-II Quantikine ELISA Kit Summary

货号:DG200

深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (25 uL)
Sensitivity
5.41 pg/mL
Assay Range
12.5 - 800 pg/mL (Serum-free Cell Culture Supern, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IGF-II.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.


Product Summary

The Quantikine® Human IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-II in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IGF-II and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGF-II.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 128 305 566 136 307 558
Standard Deviation 4.05 7.2 15.6 9.89 28.5 28.2
CV% 3.2 2.4 2.8 7.3 9.3 5.1

Recovery

The recovery of human IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum-free Cell Culture Media (n=4) 95 82-104
Urine (n=4) 94 79-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IGF-II ELISA Linearity


深圳子科生物科技有限公司
  • 联系人:苏元元女士
  • 手 机:18665335907
  • 电 话:0755-33164177
  • 地 址:广东省深圳市深圳龙华民治
  • 主 营:ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

深圳子科生物科技有限公司 地址:广东省 深圳市 深圳龙华民治 技术支持:商铺管理入口 | 产品索引 | 免责声明