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Human CNTF Quantikine ELISA Kit
深圳子科生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!
货号:DNT00
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 110 | 461 | 1295 | 128 | 399 | 821 |
| Standard Deviation | 5 | 10.2 | 31.4 | 10.1 | 26.8 | 52.6 |
| CV% | 4.5 | 2.2 | 2.4 | 7.9 | 6.7 | 6.4 |
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 103 | 406 | 1201 | 206 | 671 | 1388 |
| Standard Deviation | 4.4 | 12 | 28.2 | 19.6 | 47.8 | 93.9 |
| CV% | 4.3 | 3 | 2.3 | 9.5 | 7.1 | 6.8 |
The recovery of CNTF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 99 | 92-115 |
| Citrate Plasma (n=5) | 87 | 71-125 |
| EDTA Plasma (n=5) | 90 | 78-97 |
| Heparin Plasma (n=5) | 92 | 82-98 |
| Serum (n=5) | 95 | 85-101 |