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首页 > 精细化学品 > 生物化工 > 美国R&D > 美国R&D
  • Mouse Erythropoietin Quantikine ELISA Kit

  • Mouse Erythropoietin Quantikine ELISA Kit

  • 发布时间：2021-11-01 11:41:37
  • 关键词：Mouse Erythropoietin Quantikine ELISA Kit
  • 产品描述：Mouse Erythropoietin Quantikine ELISA Kit
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Mouse Erythropoietin Quantikine ELISA Kit Summary

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货号：MEP00B

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), Heparin Plasma (25 uL)
Sensitivity
46.9 pg/mL
Assay Range
47.0 - 3,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Heparin Plasma)
Specificity
Natural and recombinant mouse Epo. Recombinant rat Epo has 100% cross-reactivity in this kit. This kit is not validated for use with rat samples, because rat sample dilution is not linear.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse Epo Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Epo in cell culture supernates, tissue homogenates, serum, and plasma. It contains antibodies raised against recombinant Epo and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Epo showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that the Quantikine Mouse Epo kit can be used to determine relative mass values for naturally occurring mouse Epo.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 196 258 704 180 239 700
Standard Deviation 7.7 7.8 31.2 17.5 15.3 17.5
CV% 3.9 3 4.4 9.7 6.4 2.5

Recovery

The recovery of Epo spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 110 102-117
Heparin Plasma (n=8) 103 90-120
Serum (n=9) 93 85-114
Tissue Homogenates (n=3) 94 80-111

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of Epo in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse Erythropoietin ELISA Linearity
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  • 主　营：ELISA试剂盒,生化法试剂盒,分子学试剂盒,生化试剂,细胞菌株,生物培养基,实验室耗材

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