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Human CCL4/MIP-1 beta Quantikine ELISA Kit
货号：DMB00
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Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 23.3 | 98.8 | 428 | 31.4 | 118 | 476 |
| Standard Deviation | 1.1 | 3.8 | 17.3 | 3.7 | 9.9 | 28 |
| CV% | 4.7 | 3.8 | 4 | 11.8 | 8.4 | 5.9 |
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 51.3 | 208 | 932 | 58.8 | 220 | 949 |
| Standard Deviation | 4.6 | 7.5 | 30.2 | 5.7 | 15.6 | 70.5 |
| CV% | 9 | 3.6 | 3.2 | 9.7 | 7.1 | 7.4 |
The recovery of MIP-1 beta spiked to three levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 107 | 81-117 |
| Citrate Plasma (n=5) | 99 | 87-116 |
| EDTA Plasma (n=5) | 100 | 84-112 |
| Heparin Plasma (n=5) | 99 | 90-112 |
| Serum (n=5) | 99 | 88-113 |
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